Effects of cycloheximide and 5-fluorouracil on formation of low-molecular-weight ribonucleic acid in yeast.

نویسندگان

  • D V Hendricks
  • B A Andrean
  • S R De Kloet
چکیده

The effects of cycloheximide and 5-fluorouracil on the formation of low-molecular-weight ribonucleic acid (RNA) in yeast were investigated. Both compounds were found to affect the synthesis of low-molecular-weight RNA of ribosomal origin more than transfer RNA but less than the high-molecular-weight ribosomal RNA. 5-Fluorouracil-containing transfer RNA was separated from normal transfer RNA by chromatography on diethylaminoethyl cellulose at 80 C in the presence of 7 m urea.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Effects of 5-fluorouracil and 6-azauracil on the synthesis of ribonucleic acid and protein in Saccharomyces carlsbergensis.

1. Some effects of 6-azauracil and 5-fluorouracil on protein and RNA synthesis in Saccharomyces carlsbergensis were studied. 2. Both analogues caused a severe inhibition of RNA formation, whereas protein synthesis was much less affected. 3. Induced alpha-glucosidase formation was only slightly impaired. 4. Both analogues caused an inhibition of ribosome formation, although 5-fluorouracil was fa...

متن کامل

Lomofungin, an inhibitor of ribonucleic acid synthesis in yeast protoplasts: its effect on enzyme formation.

Lomofungin, an antibiotic active against fungi, yeasts, and bacteria, rapidly inhibits synthesis of ribonucleic acid (RNA) but not protein by protoplasts of Saccharomyces strain 1016. With 40 mug of lomofungin/ml, RNA synthesis was almost completely halted after 10 min of incubation; protein synthesis continued for at least 40 min. Since lomofungin inhibits isolated RNA polymerases from yeast, ...

متن کامل

Effects of Ultrasound Irradiation on the Release Profile of 5-fluorouracil from Magnetic Polylactic co-glycolic Acid Nanocapsules

Background: Drug nano-carriers are one of the most important tools for targeted cancer therapy so that undesired side effects of chemotherapy drugs are minimized. In this area, the use of ultrasound can be helpful in controlling drug release from nanoparticles to achieve higher treatment efficiency.Objective: Here, we studies the effects of ultrasound irradiation on the release profile of 5-flu...

متن کامل

A Study on Plant Polymer: Pectin Production and Modification (RESEARCH NOTE)

Abstract Pectin from two different plant sources: apple and sugar beet was studied. Apple pectinwas modified by chemical treatments such as: acid, alkali and ammonia, so as to obtain an ester levelequal to or less than 50 %, which is described as low methoxyl (LM) pectin. LM pectin form gelswith significant characteristic. In this study, de-esterification of apple pectin with acid treatmentresu...

متن کامل

Pseudohyphae formation in Candida glabrata due to CO2 exposure

Background and Purpose: Formation of pseudohyphae is considered a virulence factor in Candida species. Generally, Candida glabrata grows as budding yeast cells; however, reports illustrated that C. glabrata could form pseudohyphal cells in response to some stimuli. In this study, we provided insight into the ability of C. glabrata in forming pseudohyphal cells under different levels of carbon d...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Journal of bacteriology

دوره 97 2  شماره 

صفحات  -

تاریخ انتشار 1969